Title: Compartmentation of the Golgi complex: brefeldin-A distinguishes trans- Golgi cisternae from the trans-Golgi network Document date: 1990_9_1
ID: 47k2yobm_27
Snippet: CHO clone 13 ceils were surface labeled at 4°C and warmed for either 0 or 6 h in the presence or absence of BFA. At the end of the incubation, man6P receptors were isolated and incubated with pronase to obtain glycopeptides. Sialic acid acquisition was then determined by digestion with ~-galactosidase and gel filtration chromatography. If an oligosac-charide received sialic acid, the 3H-labeled glycopeptide would resist/~-galactosidase cleavage .....
Document: CHO clone 13 ceils were surface labeled at 4°C and warmed for either 0 or 6 h in the presence or absence of BFA. At the end of the incubation, man6P receptors were isolated and incubated with pronase to obtain glycopeptides. Sialic acid acquisition was then determined by digestion with ~-galactosidase and gel filtration chromatography. If an oligosac-charide received sialic acid, the 3H-labeled glycopeptide would resist/~-galactosidase cleavage and be excluded from Sephadex G25. In contrast, digestion of galaetose-terminating oligosaccharides would yield [3H]galactose, which would be included in the gel filtration column (10) .
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