Author: Abes, Rachida; Moulton, Hong M.; Clair, Philippe; Yang, Sung-Tae; Abes, Said; Melikov, Kamran; Prevot, Paul; Youngblood, Derek S.; Iversen, Patrick L.; Chernomordik, Leonid V.; Lebleu, Bernard
Title: Delivery of steric block morpholino oligomers by (R-X-R)(4) peptides: structure–activity studies Document date: 2008_9_16
ID: 5j496cx0_19
Snippet: Total 0.1 mg of each (R-X-R) 4 -PMO conjugate were injected in triplicate on a C18 Waters Symmetry Shield 4.6 Â 250 mm column and fitted on a Beckman-Gold HPLC chromatograph. The conjugates were eluted at a flow rate of 1 ml/min of H 2 O/0.1% TFA by a linear gradient of acetonitrile from 5% to 95% in 30 min. Elution of the conjugates was followed by UV absorption at 260 nm. Results are presented as eluting acetonitrile concentrations and express.....
Document: Total 0.1 mg of each (R-X-R) 4 -PMO conjugate were injected in triplicate on a C18 Waters Symmetry Shield 4.6 Â 250 mm column and fitted on a Beckman-Gold HPLC chromatograph. The conjugates were eluted at a flow rate of 1 ml/min of H 2 O/0.1% TFA by a linear gradient of acetonitrile from 5% to 95% in 30 min. Elution of the conjugates was followed by UV absorption at 260 nm. Results are presented as eluting acetonitrile concentrations and expressed as the mean and standard deviation of triplicate measurements.
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