Determining the impact of commercial feed additives as potential porcine epidemic diarrhea virus mitigation strategies as determined by polymerase chain reaction analysis and bioassay()

Gebhardt, Jordan T; Woodworth, Jason C; Jones, Cassandra K; Tokach, Mike D; Gauger, Philip C; Main, Rodger G; Zhang, Jianqiang; Chen, Qi; DeRouchey, Joel M; Goodband, Robert D; Stark, Charles R; Bergstrom, Jon R; Bai, Jianfa; Dritz, Steve S

An initial tissue culture 2.5 mL diluted virus inoculum (10 5 TCID 50 /mL) was inoculated into 22.5 g of gestation diet (FEED) or SDPP treated with 0.02% EO product, 0.5% BA product, combination of EO and BA products (EO + BA) (DSM Nutritional products, Parsippany, NJ), or no chemical treatment for a final infectious titer of 10 4 TCID 50 /g. The supernatant from each sample was then collected for pig bioassay on the appropriate day post-laboratory inoculation and preserved until initiation of the bioassay. The supernatant was administered one time via oral gavage on d 0 to each of three pigs per treatment (10 mL per pig). Pigs were initially 10-d old, initial BW = 3.6 kg. †(+) indicates quantifiable RNA was detected in fecal swab or cecal content as determined by qRT-PCR. (-) indicates lack of detection of quantifiable RNA in fecal swab or cecal content. Each symbol (+ or −) indicates an individual pig within bioassay room.