Determining the impact of commercial feed additives as potential porcine epidemic diarrhea virus mitigation strategies as determined by polymerase chain reaction analysis and bioassay()

Gebhardt, Jordan T; Woodworth, Jason C; Jones, Cassandra K; Tokach, Mike D; Gauger, Philip C; Main, Rodger G; Zhang, Jianqiang; Chen, Qi; DeRouchey, Joel M; Goodband, Robert D; Stark, Charles R; Bergstrom, Jon R; Bai, Jianfa; Dritz, Steve S

Following mixing of feed matrix and corresponding feed additives, 22.5 g of treated feed matrix was sampled from multiple locations within the mixer and placed in the appropriate bottle to be analyzed on 7 sampling days post-laboratory inoculation, with three replications of each sampling day per feed additive treatment combination. This process was repeated for each feed matrix × feed additive treatment combination. Both the paddle mixer and V-blender were cleaned between treatments initially by high pressure air, then a flush step was performed with either untreated FEED or SDPP for the paddle mixer and V-blender, respectively, followed by a final cleaning with high pressure air. The mixers were then prepared to mix the subsequent treatment.