Selected article for: "control serum and negative control serum"
Author: Lee, Hyojin; Kim, Eun-Ju; Song, Jae-Young; Choi, Jeong Soo; Lee, Ji Youn; Cho, In-Soo; Shin, Yeun-Kyung
Title: Development and evaluation of a competitive enzyme-linked immunosorbent assay using a monoclonal antibody for diagnosis of severe fever with thrombocytopenia syndrome virus in bovine sera
  • Document date: 2016_9_20
    • ID: 0pe8vgin_10
    Snippet: The cattle were immunized with an injection of formalininactivated SFTSV. Briefly, 60 mL of 10 8 TCID50/mL of the SFTSV was inactivated using 0.025% formalin solution for 72 h. The inactivated virus was concentrated fivefold via filtration using a 10K Ultracel (Millipore, Germany). The loss of viral infectivity was confirmed by culture in Vero cells. Two cattle aged 4 months were used to produce positive control sera against SFTSV. Approximately .....
    Document: The cattle were immunized with an injection of formalininactivated SFTSV. Briefly, 60 mL of 10 8 TCID50/mL of the SFTSV was inactivated using 0.025% formalin solution for 72 h. The inactivated virus was concentrated fivefold via filtration using a 10K Ultracel (Millipore, Germany). The loss of viral infectivity was confirmed by culture in Vero cells. Two cattle aged 4 months were used to produce positive control sera against SFTSV. Approximately 2 mL of inactivated SFTSV was emulsified in gel-based adjuvant, and the cattle received intramuscular injections every 2 weeks, with a total of three 3 injections. Serum samples were collected 2 weeks after the last antigen injection. The serum used as a negative control in IFA and cELISA was collected from cattle prior to immunization.

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