Development of a one-run real-time PCR detection system for pathogens associated with porcine respiratory diseases

SUNAGA, Fujiko; TSUCHIAKA, Shinobu; KISHIMOTO, Mai; AOKI, Hiroshi; KAKINOKI, Mari; KURE, Katsumasa; OKUMURA, Hanako; OKUMURA, Maho; OKUMURA, Atsushi; NAGAI, Makoto; OMATSU, Tsutomu; MIZUTANI, Tetsuya

By Dempo-PCR assay, multiple PRDC pathogens can be detected comprehensively and simultaneously. This assay can quickly elucidate existence of pathogens in a sample. In this study, Multiple viral and bacterial porcine respiratory pathogens were detected hyopneumoniae, M. hyorhinis) and four viruses (PCV2, PCMV, PRRS US strain, PRCV) were detected on pigs of farm F. In this study, PCMV was detected in high proportion from pigs of all farms, and mixed infection with multiple pathogens was observed. PCMV has been documented worldwide, and shows high infection rates on pig farms in Japan, Europe, North America, and China [3, 6] . Hansen et al., reported that a significant association between PCMV and PCV2 was only found in the cases of PRDC, and the role of PCMV in PRDC needs to be elucidated [7] . It is necessary to elucidate the combination of multiple pathogens for the elucidation of the etiology of PRDC, and Dempo -PCR will be a useful tool for that. PHEV is a subclinical infection, but its role as a respiratory pathogen was suggested since it was isolated from the acute respiratory disease in pigs in Michigan in 2015 [14] . The swine serological survey of PHEV also showed that it is widely and highly distributed in Japan [10] . Therefore, PHEV was added to the target pathogens of Dempo-PCR, but it was not detected from these samples.