Author: Lyoo, Kwang-Soo; Yeom, Minjoo; Kim, Jungho; Kim, Donghyuk; Ha, Gunwoo; Na, Woonsung; Le, Van Phan; Song, Daesub
Title: Development of rapid immunochromatographic strip test for the detection of porcine epidemic diarrhoea virus Document date: 2017_12_2
ID: 4szmu1dh_18
Snippet: Of the 130 swab samples tested, 58 were positive for PEDV and 72 were negative according to the ICA kit, whereas 60 were positive and 70 were negative according to real-time RT-PCR (Table 3) . Thus, three samples were positive (threshold cycle value of 29.5, 30.2 and 31.7) by real-time RT-PCR, but not detected in the ICA kit test. The relative sensitivity and specificity of the ICA kit compared with those of real-time RT-PCR were 95.0 per cent (9.....
Document: Of the 130 swab samples tested, 58 were positive for PEDV and 72 were negative according to the ICA kit, whereas 60 were positive and 70 were negative according to real-time RT-PCR (Table 3) . Thus, three samples were positive (threshold cycle value of 29.5, 30.2 and 31.7) by real-time RT-PCR, but not detected in the ICA kit test. The relative sensitivity and specificity of the ICA kit compared with those of real-time RT-PCR were 95.0 per cent (95 per cent confidence interval: 86.08-98.96) and 98.6 per cent (95 per cent confidence interval: 92.30-99.96), respectively.
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