Author: Lyoo, Kwang-Soo; Yeom, Minjoo; Kim, Jungho; Kim, Donghyuk; Ha, Gunwoo; Na, Woonsung; Le, Van Phan; Song, Daesub
Title: Development of rapid immunochromatographic strip test for the detection of porcine epidemic diarrhoea virus Document date: 2017_12_2
ID: 4szmu1dh_23
Snippet: The ICA kit has the advantages of being easy, user-friendly and deliverable without specific equipment available in field conditions. Examination of clinical samples showed that the sensitivity and specificity of the assay approached 95.0 per cent and 98.6 per cent, respectively, compared with those of the real-time RT-PCR, and 100 per cent agreement of the results were observed in faecal samples from experimentally infected piglets. Thus, the IC.....
Document: The ICA kit has the advantages of being easy, user-friendly and deliverable without specific equipment available in field conditions. Examination of clinical samples showed that the sensitivity and specificity of the assay approached 95.0 per cent and 98.6 per cent, respectively, compared with those of the real-time RT-PCR, and 100 per cent agreement of the results were observed in faecal samples from experimentally infected piglets. Thus, the ICA kit developed in this study can be used for rapid diagnosis and can assist in epidemiolog- ical surveillance of PEDV infection. The development of a rapid and easily confirmed diagnosis for PEDV antigen detection is important for controlling PED outbreaks and limiting their spread; however, the ICA kit cannot differentiate novel variant strains. Therefore, an ICA kit that can differentiate PEDV variant strains using a multiplex strip should be developed in future studies.
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