Selected article for: "IFA immunofluorescence assay and monoclonal antibody"
Author: Lyoo, Kwang-Soo; Yeom, Minjoo; Kim, Jungho; Kim, Donghyuk; Ha, Gunwoo; Na, Woonsung; Le, Van Phan; Song, Daesub
Title: Development of rapid immunochromatographic strip test for the detection of porcine epidemic diarrhoea virus
  • Document date: 2017_12_2
    • ID: 4szmu1dh_7
    Snippet: Characterisation of the monoclonal antibody was conducted by indirect immunofluorescence assay (IFA). For IFA, Vero cells inoculated with PEDV, ST cells inoculated with transmissible gastroenteritis virus (TGEV) or porcine respiratory coronavirus (PRCV), and MA-104 cells inoculated with porcine rotavirus were grown in cell culture chamber slides. The slides fixed with cold acetone were incubated with the PEDV mAbs at 37°C for 45 minutes. After w.....
    Document: Characterisation of the monoclonal antibody was conducted by indirect immunofluorescence assay (IFA). For IFA, Vero cells inoculated with PEDV, ST cells inoculated with transmissible gastroenteritis virus (TGEV) or porcine respiratory coronavirus (PRCV), and MA-104 cells inoculated with porcine rotavirus were grown in cell culture chamber slides. The slides fixed with cold acetone were incubated with the PEDV mAbs at 37°C for 45 minutes. After washing, the slides were reacted with goat antimouse IgG conjugated with FITC at 37°C for 45 minutes and examined under a fluorescence microscope.

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