Selected article for: "dna sequencing and Escherichia coli"
Author: ALFRED, Niyokwishimira; LIU, Huan; LI, Mu Lan; HONG, Shao Feng; TANG, Hai Bo; WEI, Zu Zhang; CHEN, Ying; LI, Fa Kai; ZHONG, Yi Zhi; HUANG, Wei Jian
Title: Molecular epidemiology and phylogenetic analysis of diverse bovine astroviruses associated with diarrhea in cattle and water buffalo calves in China
  • Document date: 2015_2_13
    • ID: 029hqc82_8
    Snippet: The PCR products of expected sizes were purified and then cloned into pMD18-T plasmid vectors (Takara Bio, Inc.), which were then used to transform competent Escherichia coli Top 10 cells. The recombinant plasmids were submitted in duplicate for DNA sequencing to Invitrogen Biotech (Beijing, China). Editing and assembly of nucleotide sequences were performed using the SeqMan program included in the Lasergene 7 Genomics Suite (DNAstar, Inc., Madis.....
    Document: The PCR products of expected sizes were purified and then cloned into pMD18-T plasmid vectors (Takara Bio, Inc.), which were then used to transform competent Escherichia coli Top 10 cells. The recombinant plasmids were submitted in duplicate for DNA sequencing to Invitrogen Biotech (Beijing, China). Editing and assembly of nucleotide sequences were performed using the SeqMan program included in the Lasergene 7 Genomics Suite (DNAstar, Inc., Madison, WI, U.S.A.).

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