Author: Gardner, Shea N.; Hiddessen, Amy L.; Williams, Peter L.; Hara, Christine; Wagner, Mark C.; Colston, Bill W.
Title: Multiplex primer prediction software for divergent targets Document date: 2009_9_16
ID: 7658dmvk_45
Snippet: Using the experimental conditions described in the 'Methods' section, we tested the Poxviridae 16-plex (Table 4 ) against vaccinia virus, Lister strain DNA. As assessed by agarose gel analysis, we achieved specific amplification of the predicted 617-bp amplicon for the target vaccinia genome (lane 3, Figure 7 ). The band does not appear in the no template control shown in lane 4 ( Figure 7 )......
Document: Using the experimental conditions described in the 'Methods' section, we tested the Poxviridae 16-plex (Table 4 ) against vaccinia virus, Lister strain DNA. As assessed by agarose gel analysis, we achieved specific amplification of the predicted 617-bp amplicon for the target vaccinia genome (lane 3, Figure 7 ). The band does not appear in the no template control shown in lane 4 ( Figure 7 ).
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