Author: Sultani, Mozhdeh; Mokhtari Azad, Talat; Eshragian, Mohammadreza; Shadab, Azadeh; Naseri, Maryam; Eilami, Owrang; Yavarian, Jila
Title: Multiplex SYBR Green Real-Time PCR Assay for Detection of Respiratory Viruses Document date: 2015_8_1
ID: 1dvj60yk_14
Snippet: It is notable that singleplex PCR is too arduous, expensive and sample consuming. Multiplex PCR approaches were becoming more and more acceptable for the detection of respiratory viruses, since it is cost-and timeeffective (5) . Multiplex conventional PCR has false positive results due to contamination and requires further precautions, more time and two separate machines and facilities (5) . Multiplex Taqman real-time PCR is much more expensive t.....
Document: It is notable that singleplex PCR is too arduous, expensive and sample consuming. Multiplex PCR approaches were becoming more and more acceptable for the detection of respiratory viruses, since it is cost-and timeeffective (5) . Multiplex conventional PCR has false positive results due to contamination and requires further precautions, more time and two separate machines and facilities (5) . Multiplex Taqman real-time PCR is much more expensive than the other methods. In this study, we developed a multiplex SYBR green real-time PCR for detection of 12 RNA respiratory viruses. There were 39 (22.6%) children less than six years old, in which 1 (2.56%) coronavirus NL63 was detected. Esper et al. (23) identified an incidence of 8.8% out of 895 pediatric samples positive for coronavirus NL63 annually and a French study found an incidence of 9.3% out of 300 specimens during five months (24) .
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