Author: Sultani, Mozhdeh; Mokhtari Azad, Talat; Eshragian, Mohammadreza; Shadab, Azadeh; Naseri, Maryam; Eilami, Owrang; Yavarian, Jila
Title: Multiplex SYBR Green Real-Time PCR Assay for Detection of Respiratory Viruses Document date: 2015_8_1
ID: 1dvj60yk_15
Snippet: One of the reasons for different results in detection of respiratory RNA viruses is using different clinical criteria for selection of patients in different studies. In this study, RV and EVs were not detected, which might have been due to the selection criteria, because we selected the patients hospitalized with respiratory infections, while we knew that RV and EV usually cause mild respiratory infections. We did not detect RSV which is a relati.....
Document: One of the reasons for different results in detection of respiratory RNA viruses is using different clinical criteria for selection of patients in different studies. In this study, RV and EVs were not detected, which might have been due to the selection criteria, because we selected the patients hospitalized with respiratory infections, while we knew that RV and EV usually cause mild respiratory infections. We did not detect RSV which is a relatively labile virus and might be lost by freezing and defreezing of the samples. Other limitations of our study were the small sample size and wide age range. The ability of SYBR green real-time PCR in detection of several viruses was assessed and the assay was found easy to use and cost-and time-effective, but for better understanding of sensitivity and specificity, it should be compared with other methods.
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