Title: Effect of caffeine and reduced temperature (20 degrees C) on the organization of the pre-Golgi and the Golgi stack membranes Document date: 1993_3_2
ID: 7c7slfbp_23
Snippet: These biochemical and morphological experiments suggest that in the presence of 10 mM caffeine, SFV E1 glycoproteins are retained in the ER up to 230C, whereas at 250C they reach the Golgi stack. The experiments may further indicate that SFV glycoproteins are able, at least in some degree, to escape to peripheral vesicular structures already at 240C when still possessing clearly Endo H-sensitive glycans. However, to further clarify this issue, mo.....
Document: These biochemical and morphological experiments suggest that in the presence of 10 mM caffeine, SFV E1 glycoproteins are retained in the ER up to 230C, whereas at 250C they reach the Golgi stack. The experiments may further indicate that SFV glycoproteins are able, at least in some degree, to escape to peripheral vesicular structures already at 240C when still possessing clearly Endo H-sensitive glycans. However, to further clarify this issue, morphological colocalization experiments with a marker to the pre-Golgi membranes will be necessary.
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