Author: Dong, Xiaoxv; Ni, Boran; Fu, Jing; Yin, Xingbin; You, Longtai; Leng, Xin; Liang, Xiao; Ni, Jian
Title: Emodin induces apoptosis in human hepatocellular carcinoma HepaRG cells via the mitochondrial caspase-dependent pathway Document date: 2018_8_2
ID: 02r8i5n1_21
Snippet: To investigate the effects of emodin on cell cycle distribution, HepaRG cells were stained with PI and then analyzed by flow cytometry. Compared with the control group, after 24 h of emodin (40 µM) exposure, the percentage of cells in the S and G2/M phases increased from 31.74±2.46 to 52.12±2.00% and 6.14±1.72 to 15.65±1.79%, respectively ( Fig. 6A and B) . Moreover, we investigated the expression levels of the proteins involved in the S and.....
Document: To investigate the effects of emodin on cell cycle distribution, HepaRG cells were stained with PI and then analyzed by flow cytometry. Compared with the control group, after 24 h of emodin (40 µM) exposure, the percentage of cells in the S and G2/M phases increased from 31.74±2.46 to 52.12±2.00% and 6.14±1.72 to 15.65±1.79%, respectively ( Fig. 6A and B) . Moreover, we investigated the expression levels of the proteins involved in the S and G2/M phase progression. The results demonstrated that the expression levels of p53, p21 and cyclin E proteins were significantly increased. However, other cyclin proteins (cyclin A and CDK2) were downregulated in HepaRG cells ( Fig. 6C and D) . This experiment demonstrated that emodin arrested the cell cycle at the S and G2/M phases in the HepaRG cells.
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