Author: Mohammadzadeh, Sara; Khabiri, Alireza; Roohvand, Farzin; Memarnejadian, Arash; Salmanian, Ali Hatef; Ajdary, Soheila; Ehsani, Parastoo
Title: Enhanced-Transient Expression of Hepatitis C Virus Core Protein in Nicotiana tabacum, a Protein With Potential Clinical Applications Document date: 2014_11_24
ID: 3posyr5n_19
Snippet: To detect the immuno-reactivity (antigenicity) of tobacco-derived HCVcp, an indirect ELISA for detection of anticore antibodies in human sera was developed as previously described (15) . In brief, purified pHCVcp was coated in ELISA plates (100 µL of 10 µg/mL). The pooled human sera (1/20 dilution) from five HCV seropositive patients and one serum negative (obtained from Tehran Blood Transfusion Center) were incubated with the plate for two hou.....
Document: To detect the immuno-reactivity (antigenicity) of tobacco-derived HCVcp, an indirect ELISA for detection of anticore antibodies in human sera was developed as previously described (15) . In brief, purified pHCVcp was coated in ELISA plates (100 µL of 10 µg/mL). The pooled human sera (1/20 dilution) from five HCV seropositive patients and one serum negative (obtained from Tehran Blood Transfusion Center) were incubated with the plate for two hours at room temperature. HRP-labeled anti-human IgG (1:5000 dilution, Sigma, USA) was used for detection of bound antibodies and TMB was used for color development, as described above.
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