Selected article for: "ELISA result and negative sera"
Author: Mohammadzadeh, Sara; Khabiri, Alireza; Roohvand, Farzin; Memarnejadian, Arash; Salmanian, Ali Hatef; Ajdary, Soheila; Ehsani, Parastoo
Title: Enhanced-Transient Expression of Hepatitis C Virus Core Protein in Nicotiana tabacum, a Protein With Potential Clinical Applications
  • Document date: 2014_11_24
    • ID: 3posyr5n_34
    Snippet: A) The expression level of pBI-core and PVX-core were assessed by ELISA and compared in the presence or absence of co-agroinfiltration by gene silencing suppressor P19 construct. Leaf control denotes the tobacco leaves transformed by PVX vector alone (ie, without Tr-HCVcp). B) Result of ELISA assay for confirmation of plant-derived HCVcp, using HCV-positive human serum. Negative control corresponds to HCV negative human sera. BSA was also used as.....
    Document: A) The expression level of pBI-core and PVX-core were assessed by ELISA and compared in the presence or absence of co-agroinfiltration by gene silencing suppressor P19 construct. Leaf control denotes the tobacco leaves transformed by PVX vector alone (ie, without Tr-HCVcp). B) Result of ELISA assay for confirmation of plant-derived HCVcp, using HCV-positive human serum. Negative control corresponds to HCV negative human sera. BSA was also used as another negative control. pHCVcp (corresponding to 100 µL of 10 µg/mL of pHCVcp from PVX-core-transformed tobacco leaves) and eHCVcp denote plant-and E. coli-derived HCVcp, respectively. Statistical analysis was performed by one-way analysis of variance (ANOVA), using the Bonferroni's multiple comparison test.

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