Changes in temperature alter susceptibility to a virus following a host shift

Katherine E. Roberts; Jarrod D. Hadfield; Manmohan D. Sharma; Ben Longdon

Where is the change in viral load of the i th biological replicate of host species h, 283 for temperature t (high, medium or low). β are the fixed effects, with β1 being the 284 intercepts for each temperature, β2 being the effect of basal metabolic rate, β3 the 285 effect of wing size, and β4 and β5 the effects of the critical thermal maximum (CTmax) 286 and minimum (CTmin) respectively. up are the random phylogenetic species effects 287 and e the model residuals. We also ran models that included a non-phylogenetic 288 random species effect ( !":!! ) to allow us to estimate the proportion of variation 289 explained by the host phylogeny [34,35,61]. We do not use this term in the main 290 model as we struggled to separate the phylogenetic and non-phylogenetic terms. 291