Establishment of minimal positive-control conditions to ensure brain safety during rapid development of emergency vaccines

Baek, Hyekyung; Kim, Kwang Ho; Park, Min Young; Kim, Kyeongryun; Ko, Bokyeong; Seo, Hyung Seok; Kim, Byoung Soo; Hahn, Tae-Wook; Yi, Sun Shin

From LPS(S) injection to 24 h PI, the ZO-1 mRNA level did not significantly change in B6 mice. In contrast, in ICR mice, the ZO-1 mRNA level was significantly up-and downregulated. Until 4 h PI, ZO-1 mRNA level in ICR mice progressively increased, but, at 24 h PI, the level had returned to a level similar to that at injection (0 h PI; panel A in Fig. 2 ). The occludin mRNA levels showed a decreasing tendency over 24 h in B6 mice, but the changes were not significant. In ICR mice, occludin mRNA level in early period after LPS(S) injection (i.e., 2 and 4 h PI) did not increase significantly; however, there was a notable significant increase at 24 h PI (panel B in Fig. 2 ).