Author: MINAMI, Shohei; TERADA, Yutaka; SHIMODA, Hiroshi; TAKIZAWA, Masaki; ONUMA, Mamoru; OTA, Akihiko; OTA, Yuichi; AKABANE, Yoshihito; TAMUKAI, Kenichi; WATANABE, Keiichiro; NAGANUMA, Yumiko; KANAGAWA, Eiichi; NAKAMURA, Kaneichi; OHASHI, Masanari; TAKAMI, Yoshinori; MIWA, Yasutsugu; TANOUE, Tomoaki; OHWAKI, Masao; OHTA, Jouji; UNE, Yumi; MAEDA, Ken
Title: Establishment of serological test to detect antibody against ferret coronavirus Document date: 2016_3_3
ID: 3g75spkc_8
Snippet: SDS-PAGE analysis of recombinant proteins: Purified recombinant proteins were mixed in equal volumes of 2 × sample buffer (125 mM Tris-Hcl, pH 6.8, 40% glycerol, 4% SDS, 0.002% bromophenol blue and 10% 2-mercaptoethanol) and boiled for 3 min. Samples were electrophoresed by SDS-PAGE and stained with cBB......
Document: SDS-PAGE analysis of recombinant proteins: Purified recombinant proteins were mixed in equal volumes of 2 × sample buffer (125 mM Tris-Hcl, pH 6.8, 40% glycerol, 4% SDS, 0.002% bromophenol blue and 10% 2-mercaptoethanol) and boiled for 3 min. Samples were electrophoresed by SDS-PAGE and stained with cBB.
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