Author: MINAMI, Shohei; TERADA, Yutaka; SHIMODA, Hiroshi; TAKIZAWA, Masaki; ONUMA, Mamoru; OTA, Akihiko; OTA, Yuichi; AKABANE, Yoshihito; TAMUKAI, Kenichi; WATANABE, Keiichiro; NAGANUMA, Yumiko; KANAGAWA, Eiichi; NAKAMURA, Kaneichi; OHASHI, Masanari; TAKAMI, Yoshinori; MIWA, Yasutsugu; TANOUE, Tomoaki; OHWAKI, Masao; OHTA, Jouji; UNE, Yumi; MAEDA, Ken
Title: Establishment of serological test to detect antibody against ferret coronavirus Document date: 2016_3_3
ID: 3g75spkc_9
Snippet: Quantification of recombinant proteins: concentration of purified proteins was measured using Bio-Rad Protein Assay Dye Reagent concentrate (BIO-RAD, Hercules, cA, U.S.A.) according to the manufacturer's instructions. A standard curve was constructed using bovine serum albumin (Sigma-Aldrich, St. Louis, MO, U.S.A.). The absorbance was measured using a spectrophotometer (BIO-RAD) at 595 nm......
Document: Quantification of recombinant proteins: concentration of purified proteins was measured using Bio-Rad Protein Assay Dye Reagent concentrate (BIO-RAD, Hercules, cA, U.S.A.) according to the manufacturer's instructions. A standard curve was constructed using bovine serum albumin (Sigma-Aldrich, St. Louis, MO, U.S.A.). The absorbance was measured using a spectrophotometer (BIO-RAD) at 595 nm.
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