Overexpression of Tripartite Motif Conaining 55 (TRIM55) Inhibits Migration and Invasion of Hepatocellular Carcinoma (HCC) Cells via Epithelial-Mesenchymal Transition and Matrix Metalloproteinase-2 (MMP2)

Li, Xinyu; Huang, Lei; Gao, Weijie

Data are presented as means ±standard deviation (SD). For comparisons, the t test, paired-samples t test, and Fisher's exact test were performed, as appropriate. Cumulative recurrence and survival probabilities were evaluated using the Kaplan-Meier method and Cox regression analysis, and differences were assessed using the log-rank test. P<0.05 was set as the level of significance. To investigate whether expression of TRIM55 was significantly changed during progression of HCC, we detected TRIM55 expression in 100 pairs of HCC tissues and neighboring tissues by immunohistochemistry. By using the 13-point score analysis of IHC, we found that the expression of TRIM55 in HCC tissues (76%, 76/100) was frequently lower than in neighboring tissues ( Figure 1A) . Then, we used single-factor analysis to investigate the relationship between TRIM55 expression and clinicopathologic features of HCC patients. The results showed that expression of TRIM55 was significantly associated with vessel invasion, tumor grade, and TNM stage (Table 1) . Thus, we proved that TRIM55 was reduced in HCC tissues, and lower expression of TRIM55 was associated with vessel invasion, tumor stage, and tumor grade.