Selected article for: "GenBank database and genome amplification"
Author: Won, Hokeun; Lee, Dong-Uk; Jang, Guehwan; Noh, Yun-Hee; Lee, Seung-Chul; Choi, Hwan-Won; Yoon, In-Joong; Yoo, Han Sang; Lee, Changhee
Title: Generation and protective efficacy of a cold-adapted attenuated genotype 2b porcine epidemic diarrhea virus
  • Document date: 2019_7_9
    • ID: 2hxlx1j2_12
    Snippet: The full-length genomic sequences of the parental Aram-P5 and cold-adapted Aram-P29-CA strains were determined by traditional Sanger methods. Ten overlapping cDNA fragments spanning the entire genome of each virus strain were amplified and sequenced as described elsewhere [5, 23, 24] . The 5′ and 3′ ends of the genome of the strain were determined by rapid amplification of cDNA ends (RACE) as described previously [26] . The full-length genomi.....
    Document: The full-length genomic sequences of the parental Aram-P5 and cold-adapted Aram-P29-CA strains were determined by traditional Sanger methods. Ten overlapping cDNA fragments spanning the entire genome of each virus strain were amplified and sequenced as described elsewhere [5, 23, 24] . The 5′ and 3′ ends of the genome of the strain were determined by rapid amplification of cDNA ends (RACE) as described previously [26] . The full-length genomic sequences were deposited in the GenBank database under accession numbers MK559454 through MK559456.

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