Generation and protective efficacy of a cold-adapted attenuated genotype 2b porcine epidemic diarrhea virus

Won, Hokeun; Lee, Dong-Uk; Jang, Guehwan; Noh, Yun-Hee; Lee, Seung-Chul; Choi, Hwan-Won; Yoon, In-Joong; Yoo, Han Sang; Lee, Changhee

PEDV-associated mortality occurred in > 50% piglets (11 of 20) from unvaccinated sows by 4 DPI, and additional fatalities occurred until the end of the study, indicating a rate of > 90% mortality in the unvaccinated challenge control group 2 (Fig. 8B) . In contrast, 10 of 30 piglets birthed by vaccinated sows (group 1) died from PEDV exposure, showing an approximately 70% survival rate in the piglets. PEDV fecal shedding was detected in all piglets from unvaccinated sows (group 2) by 1 or 2 DPC, and they persistently discharged high quantities (> 10 5.00 TCID 50 /mL) of PEDV in feces until death (Fig. 8C) . However, all animals from vaccinated sows (group 1) showed PEDV shedding in feces by 3 DPI with a peak mean titer of 10 5.47 TCID 50 /mL but amounts of fecal shedding significantly declined thereafter. All piglets from the negative control sow (group 3) remained alive and had no viral shedding in rectal swabs for the duration of the study.