Selected article for: "high likelihood and low score"
Author: Joana Damas; Graham M. Hughes; Kathleen C. Keough; Corrie A. Painter; Nicole S. Persky; Marco Corbo; Michael Hiller; Klaus-Peter Koepfli; Andreas R. Pfenning; Huabin Zhao; Diane P. Genereux; Ross Swofford; Katherine S. Pollard; Oliver A. Ryder; Martin T. Nweeia; Kerstin Lindblad-Toh; Emma C. Teeling; Elinor K. Karlsson; Harris A. Lewin
Title: Broad Host Range of SARS-CoV-2 Predicted by Comparative and Structural Analysis of ACE2 in Vertebrates
  • Document date: 2020_4_18
    • ID: 6ne76rh1_12
    Snippet: Comparison of vertebrate ACE2 sequences and their predicted ability to bind SARS-CoV-2 . We identified 410 unique vertebrate species with ACE2 orthologs (Dataset S1) that included representatives of all vertebrate taxonomic classes. Among these were 252 mammals, 72 birds, 65 fishes, 17 reptiles and 4 amphibians. Twenty-five amino acids corresponding to known SARS-CoV-2 S-binding residues (11, 13, 21) were examined for their similarity to the resi.....
    Document: Comparison of vertebrate ACE2 sequences and their predicted ability to bind SARS-CoV-2 . We identified 410 unique vertebrate species with ACE2 orthologs (Dataset S1) that included representatives of all vertebrate taxonomic classes. Among these were 252 mammals, 72 birds, 65 fishes, 17 reptiles and 4 amphibians. Twenty-five amino acids corresponding to known SARS-CoV-2 S-binding residues (11, 13, 21) were examined for their similarity to the residues in human ACE2 (Fig. 1, Dataset S1 ). On the basis of 4 known interactions between specific residues on ACE2 and the RBD of SARS-CoV-2 S, a set of rules was developed for predicting the likelihood of S binding to ACE2 from each species (see Materials and Methods). Five score categories were predicted: very high , high , medium , low and very low . Results for all species and all SARS-CoV-2 S binding scores are shown in Dataset S1, and results for mammalian species are also shown in Fig. 1 . The very high classification had at least 23/25 ACE2 residues identical to their human homolog and other constraints on substitutions at SARS-CoV-2 S binding hot spots (see Materials and Methods). The 18 species predicted as very high were all Old World primates and apes completely identical to human across the 25 ACE2 binding residues. The ACE2 proteins of 28 species were classified as having a high likelihood of binding the S RBD. Among them are twelve cetaceans, seven rodents, three cervids (deer), three lemuriform primates, two representatives of the order Pilosa (Giant anteater and Southern tamandua), and one Old World primate (Angola colobus, Fig. 1 ).

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