Author: Wang, Yuan; Li, Yan; Ding, Tianbing
Title: Heat shock protein 90ß in the Vero cell membrane binds Japanese encephalitis virus Document date: 2017_6_26
ID: 7cpxg1b4_33
Snippet: To elucidate the mechanisms through which HSP90β influences JEV infection in Vero cells, siRNA technology was used to knock down HSP90β expression instead of functional inhibitors, such as geldanamycin, which interferes with HSP90β ATPase activity (46) . Several siRNA fragments directed to the human HSP90β mRNA sequence were synthesised and transfected into Vero cells via lentiviral vectors. We only selected out one transfected cell line with.....
Document: To elucidate the mechanisms through which HSP90β influences JEV infection in Vero cells, siRNA technology was used to knock down HSP90β expression instead of functional inhibitors, such as geldanamycin, which interferes with HSP90β ATPase activity (46) . Several siRNA fragments directed to the human HSP90β mRNA sequence were synthesised and transfected into Vero cells via lentiviral vectors. We only selected out one transfected cell line with over 50% steady inhibition of HSP90β expression (Fig. 4A) , whose siRNA sequence is identical to an effective one reported earlier that corresponds to human HSP90β nt 1012-1030 (31) . The plaque formation assay results indicated that cells with reduced HSP90β expression produced significantly less JEV progeny, particularly at 24 and 36 hpi (5.6-and 6.5-fold less, respectively).
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