Impact of dietary spray-dried bovine plasma addition on pigs infected with porcine epidemic diarrhea virus

Duffy, Mark A; Chen, Qi; Zhang, Jianqiang; Halbur, Patrick G; Opriessnig, Tanja

For the PEDV inoculation, the passage 7 PEDV G2b isolate 13-19338E (Chen et al., 2014) at viral concentration of 10 3.0 50% tissue culture infectious dose per milliliter was used. In brief, PEDV was propagated on Vero cells (ATCC CCL-81) with minimal essential medium supplemented with tryptose phosphate broth (0.3%), yeast extract (0.02%), trypsin 250 (5 µg/mL), gentamicin (0.05 mg/mL), penicillin (100 unit/mL), streptomycin (100 µg/ mL), and amphotericin (0.25 µg/mL) as previously described (Chen et al., 2014) . Commercial liquid porcine plasma negative for PEDV RNA was used as diluent to adjust the PEDV inoculum (PEDV and PEDV-BovSDP groups) or saline (negative control [NEG] group) to a total volume of 10 mL for each pig. Inoculation was performed orally by the same team for all pigs. The pigs were held in vertical recumbence between the legs of the holder while the second person administered the material by slowly dripping the inoculum into the mouth of the pig using a syringe on dpi 0 when the pigs were 3 wk old.