Selected article for: "Cell lysate and lysis buffer"
Author: Yen, Wei-Chen; Wu, Yi-Hsuan; Wu, Chih-Ching; Lin, Hsin-Ru; Stern, Arnold; Chen, Shih-Hsiang; Shu, Jwu-Ching; Tsun-Yee Chiu, Daniel
Title: Impaired inflammasome activation and bacterial clearance in G6PD deficiency due to defective NOX/p38 MAPK/AP-1 redox signaling
  • Document date: 2019_11_2
    • ID: 6fw4thkq_20
    Snippet: G6PD activity was measured spectrophotometrically at 340 nm by the reduction of NADP + in the presence of glucose-6-phosphate as previously described [38] . In brief, the cells were lysed in lysis buffer (50 mM Tris-HCl, 150 mM NaCl 2 , 1 mM EDTA, 0.05% SDS, 1 mM NaF, 1% Triton-X 100, pH 7.5). After centrifugation, the cell lysate was reacted with G6PD assay buffer (50 mM Tris-HCl (pH 8), 50 mM MgCl 2 , 4 mM NADP + , and 4 mM glucose 6-phosphate).....
    Document: G6PD activity was measured spectrophotometrically at 340 nm by the reduction of NADP + in the presence of glucose-6-phosphate as previously described [38] . In brief, the cells were lysed in lysis buffer (50 mM Tris-HCl, 150 mM NaCl 2 , 1 mM EDTA, 0.05% SDS, 1 mM NaF, 1% Triton-X 100, pH 7.5). After centrifugation, the cell lysate was reacted with G6PD assay buffer (50 mM Tris-HCl (pH 8), 50 mM MgCl 2 , 4 mM NADP + , and 4 mM glucose 6-phosphate). The G6PD activity was analyzed at 340 nm by spectrophotometry (Beckman Coulter, USA).

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