Author: Yen, Wei-Chen; Wu, Yi-Hsuan; Wu, Chih-Ching; Lin, Hsin-Ru; Stern, Arnold; Chen, Shih-Hsiang; Shu, Jwu-Ching; Tsun-Yee Chiu, Daniel
Title: Impaired inflammasome activation and bacterial clearance in G6PD deficiency due to defective NOX/p38 MAPK/AP-1 redox signaling Document date: 2019_11_2
ID: 6fw4thkq_28
Snippet: Cells were incubated for 30 min with 10 μM CM-H 2 DCFDA (Thermo Fisher Scientific, MA, USA) at 37°C. The cells were detached from the wells by a 5 min incubation with PBS (containing 50 mM EDTA) and a 5 min incubation with Trypsin-EDTA at 37°C. The cells were washed twice and re-suspended in PBS (containing 1% FBS) before flow cytometry (FCM) analysis. FCM data are presented as the mean fluorescence intensity (MFI) for all cells......
Document: Cells were incubated for 30 min with 10 μM CM-H 2 DCFDA (Thermo Fisher Scientific, MA, USA) at 37°C. The cells were detached from the wells by a 5 min incubation with PBS (containing 50 mM EDTA) and a 5 min incubation with Trypsin-EDTA at 37°C. The cells were washed twice and re-suspended in PBS (containing 1% FBS) before flow cytometry (FCM) analysis. FCM data are presented as the mean fluorescence intensity (MFI) for all cells.
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