Author: Jung, Juwon; Baek, Jin Ah; Seol, Hye Won; Choi, Young Min
Title: Propagation of Human Embryonic Stem Cells on Human Amniotic Fluid Cells as Feeder Cells in Xeno-Free Culture Conditions Document date: 2016_3_23
ID: 20xe4vm6_10
Snippet: After 5-6 days of subculture, SNUhES32 and H1 were washed with dPBS (Invitrogen) and fixed with 4% parafor-maldehyde (PFA; Usb). AP expression was examined by staining using an Alkaline Phosphatase diagnostic kit (Sigma) according to the manufacture's instructions......
Document: After 5-6 days of subculture, SNUhES32 and H1 were washed with dPBS (Invitrogen) and fixed with 4% parafor-maldehyde (PFA; Usb). AP expression was examined by staining using an Alkaline Phosphatase diagnostic kit (Sigma) according to the manufacture's instructions.
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