Detection of kobuvirus RNA in Japanese domestic dogs

SOMA, Takehisa; MATSUBAYASHI, Makoto; SASAI, Kazumi

In the period from 2012 to 2015, fecal samples were collected from a total of 94 household (privately owned) dogs with diarrhea (37, 6, 25 and 26 samples in 2012, 2013, 2014 and 2015, respectively), which visited veterinary clinics nationwide in Japan, in which the involvement of CPV-2 infection was ruled out by PCR (mentioned later). Furthermore, the fecal samples obtained from 50 clinically healthy kenneled dogs, aged 1-3 months, were collected from 4 pet shops in Japan. The sample was suspended in a sterile saline, the suspension was centrifuged, and nucleic acid was isolated from the supernatant using a QIAamp Viral RNA Mini Kit (Qiagen science, Germantown, MD, U.S.A.), following the manufacturer's instruction. The extract was preserved at −45°C until used.