Author: Gebhardt, Jordan T; Woodworth, Jason C; Jones, Cassandra K; Tokach, Mike D; Gauger, Philip C; Main, Rodger G; Zhang, Jianqiang; Chen, Qi; DeRouchey, Joel M; Goodband, Robert D; Stark, Charles R; Bergstrom, Jon R; Bai, Jianfa; Dritz, Steve S
Title: Determining the impact of commercial feed additives as potential porcine epidemic diarrhea virus mitigation strategies as determined by polymerase chain reaction analysis and bioassay() Document date: 2018_8_20
ID: 6rlbiukh_11
Snippet: Inoculation was carried out at the Kansas State University College of Veterinary Medicine Virology Laboratory. The viral inoculum was cell culture derived USA/IN/2013/19338, passage 8, and had an initial concentration of 10 6 Tissue Culture Infectious Dose (TCID) 50 /mL. Fifty milliliters of concentrated inoculum was mixed with 450 mL tissue culture media, resulting in a diluted inoculum concentration of 10 5 TCID 50 /mL. Inoculation occurred by .....
Document: Inoculation was carried out at the Kansas State University College of Veterinary Medicine Virology Laboratory. The viral inoculum was cell culture derived USA/IN/2013/19338, passage 8, and had an initial concentration of 10 6 Tissue Culture Infectious Dose (TCID) 50 /mL. Fifty milliliters of concentrated inoculum was mixed with 450 mL tissue culture media, resulting in a diluted inoculum concentration of 10 5 TCID 50 /mL. Inoculation occurred by pipetting 2.5 mL diluted viral inoculum into each bottle containing 22.5 g treated feed matrix, resulting in an inoculated feed matrix with a viral concentration of 10 4 TCID 50 /g of feed matrix. Following addition of the viral inoculum to each bottle, the bottles were lightly shaken in a circular pattern for approximately 5 s, after which each bottle was hand-shaken and inverted for approximately 10 s to mix the virus evenly within each bottle.
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