Determining the impact of commercial feed additives as potential porcine epidemic diarrhea virus mitigation strategies as determined by polymerase chain reaction analysis and bioassay()

Gebhardt, Jordan T; Woodworth, Jason C; Jones, Cassandra K; Tokach, Mike D; Gauger, Philip C; Main, Rodger G; Zhang, Jianqiang; Chen, Qi; DeRouchey, Joel M; Goodband, Robert D; Stark, Charles R; Bergstrom, Jon R; Bai, Jianfa; Dritz, Steve S

The d 0 FEED treated with EO and BA pigs (3/3) were shedding PEDV RNA as detected by fecal swabs beginning on 2 dpi and remained infected through necropsy at 7 dpi. Virus shedding was observed 2 dpi in fecal swabs in one pig gavaged with d 7 postinoculation SDPP sample treated with both EO and BA, and all three pigs were shedding virus at 6 dpi and had virus detectible in cecal contents at necropsy. None of the FEED treated with both EO and BA had detectible RNA in fecal swabs or cecal contents with the exception of d 0 post-laboratory inoculation samples. The combination of EO and BA enhanced degradation of PEDV RNA in swine feed but had no impact on RNA degradation in SDPP. Furthermore, both untreated feed and feed treated with the combination of EO and BA resulted in PEDV infection at d 0 post-laboratory inoculation; however, neither set of samples were infective at d 1 post-laboratory inoculation.