Author: SUNAGA, Fujiko; TSUCHIAKA, Shinobu; KISHIMOTO, Mai; AOKI, Hiroshi; KAKINOKI, Mari; KURE, Katsumasa; OKUMURA, Hanako; OKUMURA, Maho; OKUMURA, Atsushi; NAGAI, Makoto; OMATSU, Tsutomu; MIZUTANI, Tetsuya
Title: Development of a one-run real-time PCR detection system for pathogens associated with porcine respiratory diseases Document date: 2019_12_23
ID: 0pkbbb99_8
Snippet: To verify the sensitivity, linearity, and efficiency of the real-time PCR assay, the limit of detection (LOD), correlation coefficient (R 2 ) and PCR efficiency (E) were determined from standard curves. Standard curves were obtained, and the LOD, R 2 and E were calculated as described previously [11, 26] ......
Document: To verify the sensitivity, linearity, and efficiency of the real-time PCR assay, the limit of detection (LOD), correlation coefficient (R 2 ) and PCR efficiency (E) were determined from standard curves. Standard curves were obtained, and the LOD, R 2 and E were calculated as described previously [11, 26] .
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