Protective Effect and Mechanism of Alprostadil in Acute Respiratory Distress Syndrome Induced by Oleic Acid in Rats

Yan, Xiujuan; Li, Yingxiu; Choi, Yun Ho; Wang, Chongyang; Piao, Yihua; Ye, Jing; Jiang, Jingzhi; Li, Liangchang; Xu, Huixian; Cui, Qingsong; Yan, Guanghai; Jin, Minggen

The rats were randomly divided into 5 groups (n=6): control, OA (Bai Hui Biological Technology Co., Ltd, Shenyang, China) model, OA + Alprostadil (2.5 μg/kg), OA + Alprostadil (5 μg/kg), and OA + Alprostadil (10 μg/kg) groups. The dose of alprostadil was determined according to previous reports [31, 32] . After the rats were anesthetized with 20% urethane (Bai Hui Biological Technology Co., Ltd.) at a dose of 1 g/kg, a dose of 0.1 ml/kg OA and an equal amount of saline were injected slowly into the femoral vein at to induce ARDS. Rats in the control group were administered an equal volume of saline. Immediately after OA administration, alprostadil injection (Beijing Tide Pharmaceutical Co., Ltd., China national medicine permission number: H01980024) at the dose of 2.5, 5, or 10 μg/kg and an equal amount of saline were given administered to rats in the OA + Alprostadil (2.5 μg/kg), OA + Alprostadil (5 μg/kg), OA + Alprostadil (10 μg/kg) groups, respectively, by femoral vein injection. Three hours after OA administration, all animals were sacrificed and the lungs were excised.