Author: Munday, Diane C.; Emmott, Edward; Surtees, Rebecca; Lardeau, Charles-Hugues; Wu, Weining; Duprex, W. Paul; Dove, Brian K.; Barr, John N.; Hiscox, Julian A.
Title: Quantitative Proteomic Analysis of A549 Cells Infected with Human Respiratory Syncytial Virus Document date: 2010_7_20
ID: 2zhaknbi_19
Snippet: Mitochondrial Transition Pore Assay and Live Cell Imaging-Glass bottom plate-and coverslip-adhered A549 cell monolayers were infected with HRSV at an m.o.i. of 2 or mock-infected. The plates were rocked periodically at 37°C for 2 h before the inoculum was replaced with fresh growth media and the plates were incubated at 37°C for a further 24 h. The ImageiT TM LIVE Mitochondrial Transition Pore Assay kit (I35103) obtained from Molecular Probes (.....
Document: Mitochondrial Transition Pore Assay and Live Cell Imaging-Glass bottom plate-and coverslip-adhered A549 cell monolayers were infected with HRSV at an m.o.i. of 2 or mock-infected. The plates were rocked periodically at 37°C for 2 h before the inoculum was replaced with fresh growth media and the plates were incubated at 37°C for a further 24 h. The ImageiT TM LIVE Mitochondrial Transition Pore Assay kit (I35103) obtained from Molecular Probes (Invitrogen) was used (according to the manufacturer's instructions) to visualize the mitochondrial transition pore in HRSV-infected, mock-infected, and positive control cells.
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