Selected article for: "MERS cov and RNA extraction"
Author: Kim, Mi-Na; Ko, Young Jin; Seong, Moon-Woo; Kim, Jae-Seok; Shin, Bo-Moon; Sung, Heungsup
Title: Analytical and Clinical Validation of Six Commercial Middle East Respiratory Syndrome Coronavirus RNA Detection Kits Based on Real-Time Reverse-Transcription PCR
  • Document date: 2016_6_24
    • ID: 1le537yd_35
    Snippet: The positivity rates for kits when testing the high inhibition panel correlated with their analytical sensitivities, as only PowerChek detected 50% of known-positive specimens. Based on these findings, it is clear that clinical sensitivity can be significantly affected by the presence of PCR inhibitors, and analytical sensitivity may be affected by PCR inhibition-prone specimens such as sputum specimens. Therefore, optimization of the pretreatmen.....
    Document: The positivity rates for kits when testing the high inhibition panel correlated with their analytical sensitivities, as only PowerChek detected 50% of known-positive specimens. Based on these findings, it is clear that clinical sensitivity can be significantly affected by the presence of PCR inhibitors, and analytical sensitivity may be affected by PCR inhibition-prone specimens such as sputum specimens. Therefore, optimization of the pretreatment and RNA extraction procedures is necessary to improve the sensitivity of MERS-CoV molecular diagnostics, especially for testing sputum specimens.

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