Author: Song, Jae-Hyoung; Shim, Aeri; Kim, Yeon-Jeong; Ahn, Jae-Hee; Kwon, Bo-Eun; Pham, Thuy Trang; Lee, Jongkook; Chang, Sun-Young; Ko, Hyun-Jeong
Title: Antiviral and Anti-Inflammatory Activities of Pochonin D, a Heat Shock Protein 90 Inhibitor, against Rhinovirus Infection Document date: 2018_5_2
ID: 0bwf8f1i_16
Snippet: We performed ELISA for assessing the cytokine and chemokine levels. ELISA kits for TNF-α, IL-1β, and CCL2 (MCP1) were purchased from e-Bioscience, and the ELISA kit for CXCL1 was purchased from R&D Systems. Bronchoalveolar lavage fluid (BALF) was obtained as described previously (Seo et al., 2010) . Lungs from mice infected with HRV1B were dissected and homogenized. The levels of cytokines and chemokines in the lung homogenates were evaluated a.....
Document: We performed ELISA for assessing the cytokine and chemokine levels. ELISA kits for TNF-α, IL-1β, and CCL2 (MCP1) were purchased from e-Bioscience, and the ELISA kit for CXCL1 was purchased from R&D Systems. Bronchoalveolar lavage fluid (BALF) was obtained as described previously (Seo et al., 2010) . Lungs from mice infected with HRV1B were dissected and homogenized. The levels of cytokines and chemokines in the lung homogenates were evaluated according to the manufacturer's instructions (Seo et al., 2011) . Absorbance was then read at 450 nm using SPECTRA MAX 340 (Molecular Devices).
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