Selected article for: "colorimetric detection and LAMP reaction"
Author: Draz, Mohamed Shehata; Shafiee, Hadi
Title: Applications of gold nanoparticles in virus detection
  • Document date: 2018_2_15
    • ID: 1xjmlwqr_101
    Snippet: Based on the color change of AuNPs upon aggregation, AuNPs carrying HPV DNA probe were coupled with the LAMP technique for colorimetric detection of HPV. LAMP reaction was performed at a temperature of 65°C for 20 min and the generated products were hybridized with AuNP-DNA probes for 5 min. Then the presence of the target DNA was detected by the addition of magnesium salt. The addition of the salt causes AuNPs aggregation and the color changes .....
    Document: Based on the color change of AuNPs upon aggregation, AuNPs carrying HPV DNA probe were coupled with the LAMP technique for colorimetric detection of HPV. LAMP reaction was performed at a temperature of 65°C for 20 min and the generated products were hybridized with AuNP-DNA probes for 5 min. Then the presence of the target DNA was detected by the addition of magnesium salt. The addition of the salt causes AuNPs aggregation and the color changes from red to blue. The presence of LAMP amplicons can protect AuNPs from aggregation and prevents AuNPs aggregation. The sensitivity of the developed LAMP-AuNP assay was greater than the LAMP turbidity assay by up to 10-fold with detection limits of 10 2 and 10 0 copies for HPV16 and HPV18, respectively [27] .

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