Author: Draz, Mohamed Shehata; Shafiee, Hadi
Title: Applications of gold nanoparticles in virus detection Document date: 2018_2_15
ID: 1xjmlwqr_63
Snippet: target concentration [65] . Furthermore, Lu et al. developed a fluorometric assay for HBV DNA detection in which positively charged AuNRs are employed to chelate fluorescein amidite (FAM)tagged ssDNA electrostatically onto their surface, forming FAM-ssDNA-AuNR ternary complexes [61] . The formation of these complex results in the FRET-based quenching of FAM fluorescence, and when complementary target DNA is present in the system, a FAM-ssDNA/cDNA.....
Document: target concentration [65] . Furthermore, Lu et al. developed a fluorometric assay for HBV DNA detection in which positively charged AuNRs are employed to chelate fluorescein amidite (FAM)tagged ssDNA electrostatically onto their surface, forming FAM-ssDNA-AuNR ternary complexes [61] . The formation of these complex results in the FRET-based quenching of FAM fluorescence, and when complementary target DNA is present in the system, a FAM-ssDNA/cDNA duplex is formed. This DNA duplex is comparatively more negative than the FAM-ssDNA and can mediate stronger electrostatic interactions with AuNRs, leading to increased FRET efficiency, which manifests as a further decline in the fluorescence intensity of FAM. This change in fluorescence intensity is utilized to sense and estimate the exact concentration of the added target DNA (Fig. 9C) .
Search related documents:
Co phrase search for related documents- complementary target dna and electrostatic interaction: 1
- complementary target dna and fluorometric assay: 1
- complementary target dna and target dna: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16
- complex result and ternary complex: 1
- dna detection and electrostatic interaction: 1
- dna detection and fluorescence intensity: 1, 2, 3, 4, 5, 6, 7
- dna detection and fluorometric assay: 1
- dna detection and target concentration: 1, 2, 3, 4, 5, 6, 7, 8
- dna detection and target dna: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73
- dna duplex and target dna: 1, 2
- dna duplex and ternary complex: 1
Co phrase search for related documents, hyperlinks ordered by date