Author: Draz, Mohamed Shehata; Shafiee, Hadi
Title: Applications of gold nanoparticles in virus detection Document date: 2018_2_15
ID: 1xjmlwqr_63
Snippet: target concentration [65] . Furthermore, Lu et al. developed a fluorometric assay for HBV DNA detection in which positively charged AuNRs are employed to chelate fluorescein amidite (FAM)tagged ssDNA electrostatically onto their surface, forming FAM-ssDNA-AuNR ternary complexes [61] . The formation of these complex results in the FRET-based quenching of FAM fluorescence, and when complementary target DNA is present in the system, a FAM-ssDNA/cDNA.....
Document: target concentration [65] . Furthermore, Lu et al. developed a fluorometric assay for HBV DNA detection in which positively charged AuNRs are employed to chelate fluorescein amidite (FAM)tagged ssDNA electrostatically onto their surface, forming FAM-ssDNA-AuNR ternary complexes [61] . The formation of these complex results in the FRET-based quenching of FAM fluorescence, and when complementary target DNA is present in the system, a FAM-ssDNA/cDNA duplex is formed. This DNA duplex is comparatively more negative than the FAM-ssDNA and can mediate stronger electrostatic interactions with AuNRs, leading to increased FRET efficiency, which manifests as a further decline in the fluorescence intensity of FAM. This change in fluorescence intensity is utilized to sense and estimate the exact concentration of the added target DNA (Fig. 9C) .
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