Author: Li, Yuetao; Zhao, Yongkun; Wang, Cuiling; Zheng, Xuexing; Wang, Hualei; Gai, Weiwei; Jin, Hongli; Yan, Feihu; Qiu, Boning; Gao, Yuwei; Li, Nan; Yang, Songtao; Xia, Xianzhu
Title: Packaging of Rift Valley fever virus pseudoviruses and establishment of a neutralization assay method Document date: 2018_3_23
ID: 4nphwznx_17
Snippet: Healthy mouse serum was used as a negative control. The antibody and RVFV/vesicular stomatitis virus (VSV)-G pseudovirus solution (500 IU) were mixed at equal volumes, added to 96-well cell culture plates, and incubated at 37 o C for 1 h. Next, the mixture was added to 293T cells in the logarithmic growth phase at 100 μL/well (containing 5 × 10 4 cells) and mixed thoroughly. After the cells were cultured at 37 o C with 5% CO2 for 72 h, the numb.....
Document: Healthy mouse serum was used as a negative control. The antibody and RVFV/vesicular stomatitis virus (VSV)-G pseudovirus solution (500 IU) were mixed at equal volumes, added to 96-well cell culture plates, and incubated at 37 o C for 1 h. Next, the mixture was added to 293T cells in the logarithmic growth phase at 100 μL/well (containing 5 × 10 4 cells) and mixed thoroughly. After the cells were cultured at 37 o C with 5% CO2 for 72 h, the number of cells with positive fluorescence in each well was determined via fluorescence microscopy.
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