Author: Li, Yuetao; Zhao, Yongkun; Wang, Cuiling; Zheng, Xuexing; Wang, Hualei; Gai, Weiwei; Jin, Hongli; Yan, Feihu; Qiu, Boning; Gao, Yuwei; Li, Nan; Yang, Songtao; Xia, Xianzhu
Title: Packaging of Rift Valley fever virus pseudoviruses and establishment of a neutralization assay method Document date: 2018_3_23
ID: 4nphwznx_6
Snippet: To enhance expression, codon optimization was performed on the G-protein gene sequence of the RVFV ZH501 strain. The cloning primers were designed based on optimized sequences, and Not I and Kpn I restriction sites were introduced at the 5´ ends of the upstream (5´-CGGGGTACCATGGCTGGTATC GCTATGAC-3´) and downstream (5´-ATTTGCGGCCGCTT AAGAGGCTTTCTTTGTGGC-3´) primers. The primers were synthesized by Sangon Biotech (China)......
Document: To enhance expression, codon optimization was performed on the G-protein gene sequence of the RVFV ZH501 strain. The cloning primers were designed based on optimized sequences, and Not I and Kpn I restriction sites were introduced at the 5´ ends of the upstream (5´-CGGGGTACCATGGCTGGTATC GCTATGAC-3´) and downstream (5´-ATTTGCGGCCGCTT AAGAGGCTTTCTTTGTGGC-3´) primers. The primers were synthesized by Sangon Biotech (China).
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