Author: Lew, Qiao Jing; Chu, Kai Ling; Lee, Jialing; Koh, Poh Ling; Rajasegaran, Vikneswari; Teo, Jin Yuan; Chao, Sheng-Hao
Title: PCAF interacts with XBP-1S and mediates XBP-1S-dependent transcription Document date: 2010_9_4
ID: 174q2pjw_18
Snippet: ( Figure 1A ). The anti-XBP-1 antibody used in the assays can recognize both XBP-1 isoforms. The association between PCAF and another member of CREB/ATF protein family, CREB1, was also examined ( Figure 1A ). PCAF was found in the immunoprecipitated complexes of XBP-1S expressing cells, but not in XBP-1U or CREB1 expressing cells ( Figure 1A ). Reciprocal IP was carried out using an anti-PCAF antibody and XBP-1S was detected in the immunoprecipit.....
Document: ( Figure 1A ). The anti-XBP-1 antibody used in the assays can recognize both XBP-1 isoforms. The association between PCAF and another member of CREB/ATF protein family, CREB1, was also examined ( Figure 1A ). PCAF was found in the immunoprecipitated complexes of XBP-1S expressing cells, but not in XBP-1U or CREB1 expressing cells ( Figure 1A ). Reciprocal IP was carried out using an anti-PCAF antibody and XBP-1S was detected in the immunoprecipitated complexes, confirming the interaction between PCAF and XBP-1S ( Figure 1B) . Interaction between XBP-1S and another HAT, p300, was examined next. However, no association between p300, XBP-1S, XBP-1U and CREB1 was detected ( Figure 1C) , indicating a specific binding between PCAF and XBP-1S.
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