2016 ACVIM Forum Research Abstract Program

A total of 17 dogs of various breeds and age with CKD (n = 6 in IRIS stage 2; n = 11 in IRIS stage 3) that were being fed a renal diet, and 15 healthy dogs of different breeds and age, being fed a maintenance diet, were included. Dogs with CKD were followed for up to 12 months or until death. A human-specific FGF-23 ELISA was validated for this study, showing linearity and 2.8% and 8.8% intra and inter-assay coefficients of variation, respectively. Serum FGF-23 concentrations were higher in CKD dogs in stages 2 and 3 on presentation (2,605.4 AE 872.0 pg/mL; meanAESEM) compared to healthy dogs (258.7 AE 21.2 pg/mL); significant difference was detected between healthy versus CKD dogs in stage 3 (P < 0.01;one-way ANOVA), and stage 2 versus stage 3 (P < 0.05). On presentation, all dogs in stage 2 CKD were normophosphatemic (3.8 AE 0.2 mg/dL), serum FGF-23 was 499.1 AE 135.8 and 3/6 dogs showed increased FGF-23 concentrations (505.5 -1,065.5 pg/mL), and there was no correlation between FGF-23 and phosphorus concentrations on admission and follow up (r = 0.286; P = 0.1185).