2016 ACVIM Forum Research Abstract Program

In conclusion, serum FGF-23 could be used as an early marker of phosphorus dysregulation in dogs with CKD as increased FGF-23 concentrations were found in normophosphatemic CKD dogs in stages 2 and 3. More marked elevated serum levels of FGF-23 were found in advanced renal disease -IRIS stage 3. It is likely that increased concentrations of FGF-23 contribute to disturbed calcium and phosphorus homeostasis and altered vitamin D metabolism and parathyroid gland function. Further studies at targeted control of serum phosphorous and FGF23 concentrations in dogs with CKD are warranted. Idiopathic cystitis (IC) is a common lower urinary tract disorder in cats that resembles painful bladder syndrome/interstitial cystitis in humans. In both disorders, the etiology is unknown and specific therapy is not available. Diagnosis is based on clinical signs and exclusion of other disorders due to lack of characteristic pathologic findings, well-defined phenotypes, or objective biomarkers. Cytokines can serve as noninvasive biomarkers to define the presence, severity, and progression of disease, and response to treatment. The objective of this pilot study was to determine concentrations of selected cytokines in serum from healthy cats and cats with IC. Thirteen serum samples from healthy cats and 13 from cats with IC were evaluated. Multiplex analysis of 19 cytokines (sFas, TNF-a, SDF-1, SCF, RANTES, PDGF-BB, MCP-1, KC, IL-18, IL-13, IL-12 (p40), IL-8, IL-6, IL-4, IL-2, IL-1b, IFN-c, GM-CSF, Flt-3L) was performed with a multiple antigen Luminex bead-based assay according to manufacturer recommendations. Results were then analyzed for normality of data with D'Agostino-Pearson test and groups were compared using Mann-Whitney test.