Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system Document date: 2019_3_18
ID: 6nq7y1qe_55
Snippet: As an alternative to leucine-zippers, we tested the functional activity of fusing vaccinia virus capping enzyme subunits to T7RNAP. The D1R or D12L ORFs were fused in-frame to the amino-terminus of T7RNAP, separated by a flexible (G 4 S) 4 linker, generating D1R-(G 4 S) 4 -T7RNAP and D12L-(G 4 S) 4 -T7RNAP constructs respectively. In comparison to uncoupled enzymes, fusion increased luciferase expression by 8-and 3.9-fold respectively ( Figure 2 .....
Document: As an alternative to leucine-zippers, we tested the functional activity of fusing vaccinia virus capping enzyme subunits to T7RNAP. The D1R or D12L ORFs were fused in-frame to the amino-terminus of T7RNAP, separated by a flexible (G 4 S) 4 linker, generating D1R-(G 4 S) 4 -T7RNAP and D12L-(G 4 S) 4 -T7RNAP constructs respectively. In comparison to uncoupled enzymes, fusion increased luciferase expression by 8-and 3.9-fold respectively ( Figure 2 ). In both approaches, coupling of D1R to T7RNAP outperformed coupling of D12L to RNAP and confirmed the importance of physically coupling capping enzyme and RNAP enzymes for T7-transcript expression.
Search related documents:
Co phrase search for related documents- enzyme subunit and functional activity: 1
- enzyme subunit and luciferase expression: 1, 2
- enzyme subunit and vaccinia virus: 1, 2
- expression enzyme and luciferase expression: 1, 2
- expression enzyme and vaccinia virus: 1, 2, 3
- functional activity and luciferase expression: 1
- leucine zipper and vaccinia virus: 1
- luciferase expression and vaccinia virus: 1, 2
Co phrase search for related documents, hyperlinks ordered by date