Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system Document date: 2019_3_18
ID: 6nq7y1qe_63
Snippet: We also tested the influence of point substitutions having predicted effects on K1ERNAP processivity. K1ERNAP amino-acids residues were selected based on conservation among the Autographivirinae family members and their known effects on in vitro processivity of T7RNAP (58) (Supplementary Figure S10 ). The R620S and I802S mutations, which were both predicted to reduce RNAP processivity, decreased gene reporter expression. In contrast, the R551S mu.....
Document: We also tested the influence of point substitutions having predicted effects on K1ERNAP processivity. K1ERNAP amino-acids residues were selected based on conservation among the Autographivirinae family members and their known effects on in vitro processivity of T7RNAP (58) (Supplementary Figure S10 ). The R620S and I802S mutations, which were both predicted to reduce RNAP processivity, decreased gene reporter expression. In contrast, the R551S mutation, which was predicted to increase polymerization kinetics, slightly increased reporter gene expression and was selected for further optimization (Supplementary Figure S11) .
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