Author: Lee, Sung-Hwan; Kim, Ha-Young; Choi, Eun Wha; Kim, Doo
Title: Causative agents and epidemiology of diarrhea in Korean native calves Document date: 2019_10_25
ID: 3xf8fjxp_15
Snippet: To detect and count Eimeria oocysts, oocysts were concentrated by fecal flotation technique at a specific gravity of 1.18 using sodium nitrate [31] . To detect Cryptosporidium spp. in feces, DNA was extracted from the fecal samples using the QIAamp Fast DNA Stool Mini Kit (Qiagen, Germany) according to the manufacturer's instructions and stored at −20°C until use. The 18S rRNA of Cryptosporidium spp. was amplified using primer set 18SiF/18SiR .....
Document: To detect and count Eimeria oocysts, oocysts were concentrated by fecal flotation technique at a specific gravity of 1.18 using sodium nitrate [31] . To detect Cryptosporidium spp. in feces, DNA was extracted from the fecal samples using the QIAamp Fast DNA Stool Mini Kit (Qiagen, Germany) according to the manufacturer's instructions and stored at −20°C until use. The 18S rRNA of Cryptosporidium spp. was amplified using primer set 18SiF/18SiR as previously described [32] . To amplify β-giardin gene fragments from Giardia duodenalis, nested PCR was performed with two primer sets G7/G759 and G7n/G759n, as previously described [33] .
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