Selected article for: "high throughput and PCR amplification"
Author: Pandya, Gagan A.; Holmes, Michael H.; Sunkara, Sirisha; Sparks, Andrew; Bai, Yun; Verratti, Kathleen; Saeed, Kelly; Venepally, Pratap; Jarrahi, Behnam; Fleischmann, Robert D.; Peterson, Scott N.
Title: A bioinformatic filter for improved base-call accuracy and polymorphism detection using the Affymetrix GeneChip® whole-genome resequencing platform
  • Document date: 2007_11_15
    • ID: 16tii0ha_44
    Snippet: The whole-genome resequencing approach described here made use of genome amplification (23, 24) , a method that has been successfully used in genotyping (25, 26) and molecular epidemiological (27) studies. The technical modification of elimination of the PCR amplification step from the resequencing procedure makes the platform more cost effective and amenable to high throughput. The resequencing array platform by nature is highly efficient. A sin.....
    Document: The whole-genome resequencing approach described here made use of genome amplification (23, 24) , a method that has been successfully used in genotyping (25, 26) and molecular epidemiological (27) studies. The technical modification of elimination of the PCR amplification step from the resequencing procedure makes the platform more cost effective and amenable to high throughput. The resequencing array platform by nature is highly efficient. A single person can easily generate whole-genome sequence and SNP data for at least four F. tularensis genomes per week without any automation using our approach. This can be further improved by technological advances in array feature density, automation and increasing the man power, providing whole-genome sequence and SNP information from multiple isolates in a matter of days.

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